Dr. Talat Roome

Research

2024:

Kawish, M., Ullah, S., Roome, T., Razzak, A., Aslam, S., & Raza Shah, M. (2024). Thermoresponsive lipids engineered magnetic nanoparticles for spatiotemporal delivery of hesperidin to inflammatory sites in animal model. Pharmaceutical Development and Technology, 29(7), 762-775. [doi: 10.1080/10837450.2024.2393216]

2023:

• Hasnain, M., Kanwal, T., Rehman, K., Rehman, S. R. U., Aslam, S., Roome, T., & Shah, M. R. Microarray needles comprised of arginine-modified chitosan/PVA hydrogel for enhanced antibacterial and wound healing potential of curcumin. International Journal of Biological Macromolecules.2023;253(1)126697. [doi:10.1016/j.ijbiomac.2023.126697].

  • The impact factor is 8.2 and HEC category “W”

• Ullah, A., Mamun, A. A., Zaidi, M. B., Roome, T., & Hasan, A. A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing. Biomedicine & pharmacotherapy. 2023, 165, 115156. [doi:10.1016/j.biopha.2023.115156].

  • The impact factor is 7.5 and HEC category “W”

• Hasan A, Roome T, Wahid M, Ansari SA, Khan JA, Kiyani A, Jilani SN. A novel experimental model to investigate fungal involvement shows expression of Dectin‐1 in periapical lesion pathogenesis. Journal of Oral Rehabilitation. 2023 June 1;50 (8). [doi:10.1111/joor.13528]

  • The impact factor is 3.558 and HEC category “W”

• Jabri T, Roome T, Razzak A, Aziz S, Imran M, Sikandar B, Elhissi A, Aslam SM, RazaShah M. Fabrication of hesperidin hybrid lecithin-folic acid silver nanoparticles and its evaluation as anti-arthritis formulation in autoimmune arthritic rat model. Journal of Molecular Structure. 2023 Mar 15;1276:134722. [doi:10.1016/j.molstruc.2022.134722]

• • The impact factor is 3.841 and HEC category “W”

• Hasan A, Roome T, Wahid M, Ansari SA, Khan JA, Jilani SN, Jawed A, Kiyani A. Expression of Toll-like receptor 2, Dectin-1, and Osteopontin in murine model of pulpitis. Clinical Oral Investigations. 2023 Mar;27(3):1177-92. [doi: 10.1007/s00784-022-04732-2]

  • The impact factor is 3.606 and HEC category “W”

2022:

• Hasan A, Roome T, Wahid M, Ansari SA, Akhtar H, Jilani SN, Kiyani A. Gene expression analysis of toll like receptor 2 and 4, Dectin-1, Osteopontin and inflammatory cytokines in human dental pulp ex-vivo. BMC Oral Health. 2022 Dec;22(1):1-5. [doi: 10.1186/s12903-022-02621-4]

  • The impact factor is 3.747 and HEC category “W”

• Aslam Z, Roome T, Razzak A, Aslam SM, Zaidi MB, Kanwal T, Sikandar B, Bertino MF, Rehman K, Shah MR. Investigation of wound healing potential of photo-active curcumin-ZnO-nanoconjugates in excisional wound model. Photodiagnosis and Photodynamic Therapy. 2022 Sep 1;39:102956. [doi: 10.1016/j.pdpdt.2022.102956]

  • The impact factor is 3.577 and HEC category “X”

2021:

• Roome T, Qasim M, Aziz S, Farooq AD, Razzaq A, Ali SF. Assessment of acute, sub-acute, chronic and genotoxicity of polyherbal formulation DCD-684 in mice. Pak. J. Pharm. Sci. 2021 Jul 1;34(4):1485-98. [PMID: 34799324]

  • The impact factor is 0.863 and HEC category “Y”

• Roome T, Qasim M, Farooq AD, Ilyas Q, Aziz S, Ali SF. Antispasmodic activity and mechanism of action of polyherbal formulation DCD-684 on rabbit jejunum. Pakistan Journal of Pharmaceutical Sciences. 2021 Mar 2;34. [PMID: 34275806]

  • The impact factor is 0.863 and HEC category “Y”

• Kanwal T, Saifullah S, ur Rehman J, Kawish M, Razzak A, Maharjan R, Imran M, Ali I, Roome T, Simjee SU, Shah MR. Design of absorption enhancer containing self-nanoemulsifying drug delivery system (SNEDDS) for curcumin improved anti-cancer activity and oral bioavailability. Journal of molecular liquids. 2021 Feb 15;324:114774. [doi: 10.1016/j.molliq.2020.114774]

  • The impact factor is 6.633 and HEC category “W”

2019:

• Roome T, Aziz S, Razzak A, Aslam Z, Jamali KS, Sikandar B, Fatima T, Abidi L, Imran M, Faizi S, Shah MR. Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: novel suppressors of toll-like receptors-2 and-4. Biomedicine & Pharmacotherapy. 2019 Apr 1;112:108624. [doi: 10.1016/j.biopha.2019.108624]

  • The impact factor is 7.419 and HEC category “Y”

2018:

• Imran M, Hameed A, Shafiullah, Hafizur RM, Ali I, Roome T, Shah MR. Fabrication of Xanthan stabilized green gold nanoparticles based tolbutamide delivery system for enhanced insulin secretion in mice pancreatic islets. Journal of Macromolecular Science, Part A. 2018 Dec 2;55(11-12):729-35. [doi: 10.1080/10601325.2018.1510290]

  • The impact factor is 2.216 and HEC category “Y”

• Rao K, Roome T, Aziz S, Razzak A, Abbas G, Imran M, Jabri T, Gul J, Hussain M, Sikandar B, Sharafat S. Bergenin loaded gum xanthan stabilized silver nanoparticles suppress synovial inflammation through modulation of the immune response and oxidative stress in adjuvant induced arthritic rats. Journal of Materials Chemistry B. 2018;6(27):4486-501. [doi: 10.1039/c8tb00672e]

  • The impact factor is 7.0 and HEC category “W”

• Rao K, Aziz S, Roome T, Razzak A, Sikandar B, Jamali KS, Imran M, Jabri T, Shah MR. Gum acacia stabilized silver nanoparticles based nano-cargo for enhanced anti-arthritic potentials of hesperidin in adjuvant induced arthritic rats. Artificial cells, nanomedicine, and biotechnology. 2018 Oct 31;46(sup1):597-607. [doi: 10.1080/21691401.2018.1431653]

  • The impact factor is 6.355 and HEC category “W”

2017:

• Zaman N, Ahmad H, Ali Abid M, Roome T, Abbas G. Acetaminophen (paracetamol) facilitated extinction learning in contextual fear conditioned rats. Letters in Drug Design & Discovery. 2017 Aug 1;14(8):898-903. [doi: 10.2174/1570180814666170203162557]

  • The impact factor is 1.099 and HEC category

BOOKS:

• Chapter “Clinical implications of metals-based drug delivery systems” in Book title; “Metals Nano-colloids: Drug Delivery and Diagnostic Applications” (In-Press).

• Chapter-3 “Concept of Disease” Talat Roome Text Book of Community Medicine and Public Health (Vol I & II) Paramount Book Publisher, Pakistan. 2017.

• Anti-inflammatory Agents of Aegiceras corniculatam: Mechanism of Action: Biochemical and Cellular Mechanism via production of Eicosanoids, Histamine, Free Radicals in vivo and in vitro systems. Lambert Academic Publishing, Germany, 2012.

PATENTS:

• A Process for Preparing Efficient and Safe Antioxidant and Analgesic Composition Containing Xanthone-C-Glucoside (Mangiferin) From Bombax Ceiba, A. Dar, Shaheen Faizi, S. Naqvi, T. Roome, S. Zikr-Ur-Rehman, M. Ali Patent No. 633/05, 2005

• Polyacrilonytrile Based Electrospun Nanofibers Loaded with Zinc Oxide Quercetin Nanoparticles for Wound Healing. Raza Shah, Tooba Jabri, Kanwal Iqbal, Talat Roome, Shafi Ullah, Qamar Khan. Publication No: US 2023/0181371 A1. June 15 2023

• Opuntiol/ Opuntioside-I: An Anti- Atherogenic Agent. Talat Roome, Shaheen Faizi, Anam Razzak, Farheen Maryam (Application in Submission under P-1 category, Patent Ordinence 2000).

• Potential Role of Novel Opuntiol/ Opuntioside-I compounds isolated from Opuntia Dillenii as immune-modulatory agents. Talat Roome, Shaheen Faizi, Perbhat Ali, Sabahat Aziz, Anam Razzak (Application in submission under P-1 category, Patent Ordinence 2000).

RESEARCH GRANTS (NATIONAL/ INTERNATIONAL):

• GRAND CHALLENGE FUND, 2022, Award under Grand Challenge Fund – (GCF-HEDP) & World Bank: “Rabies Vaccine: Development of inactivated human rabies
  vaccine against wild-type strain in Pakistan”. Reference research project No. GCF-790. Total Award & Industrial Partnership: 73,472,500.00 PKR. Duration: 2022-2025.

• PSF Grant 2023 (collaboration with AKU) “Early Detection of Oral Pre-Cancerous Lesions among Smokeless Tobacco Users Adolescents by Analyzing Salivary metabolites and Cytokines”. Reference research project No PSF/CRP/S-AKU/Consrm-(220). Total Award: 8,571,000 PKR. Duration: 2023-2025

• PC-1 Grant “Anti-snake venom Development” Agency: Government of Sindh (PC-1), Total Award Amount to DUHS: 2,383,290.00 US $. Duration: 2015-2023 (Currently in Progress)

• Research Grant-II (Collaboration between University of Aberdeen and DUHS) “Evaluation of binding of Dectin-1, Osteopontin and Vimentin and its downstream signaling in regulation of immune response and infections”

• Research Grant-IV “Single Nucleotide Polymorphism in Osteopontin Gene: In relevance to Hepatitis C Virus infection and associated Hepatocellular Carcinoma in Pakistani population”

• Research Grant-V “Development of Novel Anti-atherogenic Drug: Effect of Opuntiol and Opuntioside-I and their mechanism of action against Hyperlipidemia and Atherosclerosis in Apo-E knockout mice”

• Research Grant-X “Role of Dectin-1: A Beta Glucan Receptor in Inflammation of Endodontic Origin (Principal Investigator: Dr. Arshad Hasan, Co-Principal Investigator”

• Research Grant-XIV “Pre-clinical studies of a novel simvastatin-licithin stabilized surface coated folic acid-silver nanoformulation against atherosclerosis inApo-E knockout mice”

• Research Grant-XV “Role of 7-ketocholesterol (7-KCHO) in Pathophysiology of Diabetic Nephropathy (DN): Streptozotocin (STZ) induced Disease Model”

• Research Grant-XVI “In vitro and In vivo anticancer studies of Tagetes patula flower extract and pure compound (patuletin) against Hepatocellular carcinoma

• Research Grant-XVII “A Pre-Clinical Study: Development of a Novel SimvastatinFolic Acid Functionalized Self-Nanoemulsifying Drug Delivery Systems”

PILOT RESEARCH AND SUBMITTED GRANTS:

• “Investigating the Relationship between Stress Hormones and Different Serotypes and Genotypes of Dengue Virus”. Funding Agency: VC Seed Funding (VCSFI), Submission date: July, 2023

• “Development of Novel Gene Augmentation Therapies for Inherited Retinal Diseases”. Funding Agency: PAK-SWISS research collaboration with University Hospital Geneva, University of Geneva under VELUX STIFFTUNG, Zurich, Switzerland. Submission date: 6th May, 2023

• “Rapid Manufacturing of Antivenom to relief flood victims in Pakistan”. Funding Agency: Rapid Technology Transfer Grant (RTTG). Submission date: Feb, 2023

DUHS VENOM PROJECT BIG-4:

• Rapid Development of next generation antivenoms in Pakistan: Recombinant mAbs against Cobra species. Funding Agency: Pakistan Innovation Fund (PIF), Submission date: April, 2023.

• “Snake Venom: An innovative ingredient for Marketing as Raw Material”. Funding
  Agency: Pakistan Science Foundation (PSF), Submission date: April, 2023.

• “Hydrogel Encapsulating Snake venom derived molecules for Infectious wound healing in vivo”. Funding Agency: Pakistan Science Foundation (PSF), Submission date: April, 2023.

• “Snake Venom derived nanoconjugates: A new era of nano biomaterial-based Cancer Therapeutics”. Funding Agency: Pakistan Science Foundation (PSF), Submission date: April, 2023.

COMMERCIAL PROJECTS:

• Development & Formulation of Snake Antivenom Polyspecific Immunoglobulins

• Toxicological Studies from Pharmaceutical industries

• Preclinical Trial leading to Clinical Trials- DNA and Chloroplast Vaccine in Collaboration with DESTO BRC.

• Innovative and Collaborative Research Grant (ICRG) Project entitled “FDA approved Toxicological Studies on 2-deoxy-d-ribose alginate wound dressings” in collaboration (third party) with University of Sheffield, UK and COMSATS University, Pakistan.

• WOUND HEALING PROJECT
  ➢ Project Part A: Investigation of wound healing potential of photo-active curcumin-ZnO-nanoconjugates in excisional wound model.
  ➢ Project Part B: Polyacrylonitrile Based Electrospun Nanofibers Loaded with Zinc Oxide Quercetin Nanoparticles for Wound Healing. Collaborators are Virginia Commonwealth University USA and International Center for Chemical
  and Biological Sciences (ICCBS)

• A Randomized, Double Blind, Positive Control Study to Evaluate the Safety and Efficacy of Fuke Qianjin Capsule Administered Orally in Patients with Pelvic Inflammatory Diseases in collaboration with International Center for Chemical and Biological Sciences (ICCBS) under the project grant CPEC China

AWARDS & HONORS:

• Travel Grant awarded for the IUIS 2023: 18TH International Congress of Immunology, Cape Town, South Asia.

• 6th DUHS-DICE Health Innovation Exhibition organized on 30th December 2021 at the Ojha Campus of Dow University of Health Sciences and special prize winner for the projects entitled: ➢ Stem Cells Preconditioned with Acrinol for the Treatment of Burn Wounds. Talat Roome, Shazmeen Aslam and Midhat Batool.
  ➢ Accelerated Wound Healing by Visible Light Illumination of Nanoconjugates. Talat Roome, Midhat Batool Zaidi, Shazmeen Aslam, Salma Mirza and Anum Razzak

• International Academy of Pathology (IAP) Travel Bursary Award 2018 “Single-nucleotide Polymorphism in the Promoter Region of the Osteopontin gene as predicting marker of Hepatitis C pathogenesis and its related Hepatocellular Carcinoma in Pakistan population”.
  ➢ M. Phil Students Travel: The Late King Hussein of Jordan Bursary award for the research entitled: “Anti- atherosclerotic effect of opuntiol and its silver nanoparticle against foam cell formation via nuclear factor-KB signaling”.
  ➢ International Academy of Pathology (IAP) Travel Bursary Award 2018 for the research entitled: “Association of TLR-2 and Dectin-1 polymorphism with viral/fungal infections in liver transplant patients in Pakistan”.
  ➢ Higher Education Commission (HEC) Travel Grant Award for ORAL presentation entitled: “Berginin Loaded Xanthan stabilized silver nanoparticles prevent arthritis by suppressing TH1/TH2 cytokines and TLR2/TLR4 mRNA
  expression in adjuvant-induced arthritis” Eurasia Conference on Chemical Sciences (EuAsC2S-15), Faculty of Civil and Industrial Engineering Sapienza University of Rome, Rome, Italy, 5-8, September 2018

• USEFP- Pakistan US Alumni Network (PUAN) Alumni Grant Award for the organization of Awareness symposium and Campaign entitled “A school-based
  awareness program to improve knowledge of school going adolescents about the impact of electronic media on their health and well-being”. December, 2017-May2018.

• Best Poster Presentation Awards at APPNA-DUHS Winter Meeting 2016, 22-24 December 2016. Dow Medical College, DUHS
  • A mechanistic study to vasodilatory effect of Aegiceras corniculatum in-vivo and in-vitro models
  • Development of atherogenesis in ApoE-knockout mice: A model for novel drug screening
  • Anti-Arthritic Effect of Opuntiol via Suppressing TLRs expression in Adjuvant-Induced Arthritic Rats.

• Best Poster Presentation Award for the Research entitled: “Anti-Arthritic Effect of Opuntiol via Suppressing TLRs expression in Adjuvant-Induced Arthritic Rats” at 14th EURASIA Conference on Chemical Sciences”, ICCBS, 15-18 December, 2016.
  

• European Society of Pathology (ESP) & International Academy of Pathology (IAP) Travel Bursary Award 2016 for the abstract entitled: “Silver and Gold nanoparticle of Opuntiol: A novel suppressor of TLRs and Dectin-1 in adjuvant-induced arthritic rats”, at XXXI International Congress of the IAP and 28th Congress of the ESP held at Cologne, Germany, 25 – 29 September 2016.

• Best Poster Presentation Award for the abstract entitled “Diagnostic Significance of Plasma Osteopontin in Hepatitis C induced Hepatocellular Carcinoma in Pakistani Population” at XXXI International Congress of the IAP and 28th Congress of the ESP held at Cologne, Germany, 25 – 29 September 2016.

• USEFP – Pakistan US Alumni Network (PUAN) Alumni Grant Award for the organizationof Awareness symposium and Campaign entitled: “A school-based awareness program to improve knowledge of school children regarding association of arecanut/betel quidand chew tobacco with oral lesions” Oct-Dec 2016.

• Higher Education Commission (HEC) Travel Grant Award to Present Session Lecture “Opuntiol and Opuntioside-I inhibits MCP-1/CCR2 dependent cell migration via cPLA2ɑ and iPLA2β signaling pathways” at the 7th International Conference on Drug Discovery and Therapy, 15-18 February 2016 at the University of Sharjah, UAE.

• Dow Graduate Association of North America (DOGANA) Annual Research Award 2015 for the appreciation of research activities at national and international level, 31st December 2015.

• USEFP-Fulbright Alumni Grant (ASG) Award for the organization of awareness symposium entitled: Healthy heart healthy life: A new era of our generation A scientific and public awareness on cardiovascular disease at Department of Pathology, Dow International Medical College, Dow University of Health Sciences, 30-31st December, 2015.

• Four Fully funded Scholarship and Travel Bursary Awards on five research projects accepted for Oral presentation and Invited Lecture at 38TH Annual Conference of Pakistan Association of Pathology (PAP)/3nd Joint Conference of Societies of Pathology, November 6-8, 2015, Lahore.

• Organized Ist PSI-DUHS CME workshop entitled: “Cell Signaling in Innate Immune System” at LRC Block, Dow International Medical College, DUHS, Ojha Campus on 6th October 2015.

• Best Project Award at First All Pakistan DUHS-DICE Health Innovation Exhibition, 2015. Entitled: “Establishment of drugs and novel biomarkers screening facility: Utilizing STAM mice model resembles to human NASH”, 21-22 August 2015.

• Certificate of Appreciation from 7th Annual Research Day 2016 for representing Dow University of Health Sciences at Welcome Trust Advanced Course, Protein interactions and Networks, 3-11 August, 2015, Hinston, Cambridge, UK

• HEC NRPU Research Grant Award- (2015- 2018): “Single Nucleotide Polymorphism in Osteopontin Gene: In relevance to Hepatitis C Virus infection and associated Hepatocellular Carcinoma in Pakistani population”

• HEC NRPU Research Grant Award- (2015- 2018): “Development of Novel Anti-atherogenic Drug: Effect of Opuntiol and Opuntioside-I and their Mechanism of Action against Hyperlipidemia and Atherosclerosis in ApoE knockout mice”

• Welcome Trust Scholarship Bursary Award for Advance Course entitled “Protein Interactions and Networks” dated 3-11 August, 2015 in Hinxton, UK.

• Best Research Award in 6th Annual Inter-University Research Day of Dow University of Health Sciences, Karachi Pakistan, 28th February, 2015. “A mechanistic study to vasodilatory effect of Aegiceras corniculatum in vivo and in vitro models”

• Best Research in Biological Sciences (1st Prize in Poster competition) in 5th International Symposium-cum- Training Course on Molecular Medicine and Drug Research Karachi, Pakistan, January 12-15, 2015. The Research Awarded entitled: “Inhibitory effect of Opuntiol and Opuntioside-I against the expression of cytokines and chemokine in carrageenan and zymosan induced mouse inflammatory model”.

• Scholarship & Travel Grants from 38TH Annual Conference of Pakistan Association of Pathology (PAP)/3nd Joint Conference of Societies of Pathology, December 19-21,2014, Peshawar.

• USEFP-Fulbright Alumni Grant (ASG) Award for the organization of hands-on training workshop entitled: Evaluation of Cellular Pro-inflammatory Metabolites by Using HPLC: A Mechanistic Approach to Drug Discovery and Their Role in Diagnostics conducted at Department of Molecular Pathology, DDRRL, Dow University of Health Sciences in August 21-23, 2014.

• Secured 4 International Scholarships and Travel Awards (on 4 Research Projects; Leading Awards holder in Pakistan in 2014) from International Academy of Pathology (IAP) for Research Presentations at XXXth International Congress of Pathology, IAP 2014, Bangkok Thailand, 5-10 October 2014.

• Gold Medal Awarded for Best Research in 37TH Annual Conference of Pakistan Association of Pathology (PAP)/2nd Joint Conference of Societies of Pathology 19-21 December, 2013. Lahore. Pakistan Society of Immunology and Joint Societies of Pathology selected my Research entitled: Molecular Modeling and Docking of human Dectin-1 receptor: A novel gateway in drug discovery to control immune and inflammatory diseases.

• Gold Medal Awarded from Pakistan Society of Immunology, in Pathology Congress, December 2013, delivered on 26th April, 2014.

• Two Research selected as Best research and awarded in Inter-University Research Day of Dow University of Health Sciences, Karachi Pakistan on March 9, 2014.

• Scholarship & Travel Grant from Pakistan Association of Pathology (PAP)/2nd Joint Conference of Societies of Pathology, December 20-22, 2013, Lahore.

• Best Research in Biological Sciences (3rd Prize in Poster) under category of Rational Drug Design in 50th Anniversary Event; 14th Asian Symposium on Medicinal Plants and Other Natural Products, December 9-12, 2013. The Research Awarded entitled: Opuntiol and Opuntioside-I an anti-atherogenic agent interfering with chemotaxis via iPLA2β dependent F-actin polymerization in human monocytes.

• Charles Wallace Scholar Award for Young Scientist 2012-2013 Awarded by British Council, UK, Charles Wallace Pakistan Trust

• Charles Wallace Visiting Fellowship for 2012-2013. Fellowship for research at Institute of Medical Sciences, University of Aberdeen, UK on Cellular and Molecular basis of Immune response and infectious diseases.

• Higher Education Commission of Pakistan Award for 2011-2012 Approved as an Assistant Professor and Research Scientist under IPFP of HEC to serve
  Dow University of Health Sciences, Karachi, Pakistan.

• Higher Education Commission of Pakistan Research Grant achieved for 2011-2013 with the title of Project: Evaluation of novel strategy of anti-inflammatory agents in prevention of atherosclerosis via modulating signaling pathways involved in MCP-1 dependent monocyte chemotaxis.

• Fulbright Scholar Award for 2009-2010 Awarded by The United States Educational Foundation, US Department of States & J.William Fulbright Foreign Scholarship Board (FSB), Council of International Exchange of Scholars, Washington, USA.

• Post-doctoral Fellow Appointment for 2009-2011 Department of Cell Biology, Cleveland Clinic Lerner Research Institute, Molecular
  Medicine Program of Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland 44195, OH, USA.

• Achievement: Article on research work appreciations published in International News Letter “NEWS & NATIONS” of Cleveland Clinic Lerner Research Institute. USA 2010 (Online Article: http://www.lerner.ccf.org/news/2010/11/5.php